Hotspot

rps19-rpl2

IRb · 87441-87518 · 78 bp

Back to Ottelia

Pi 0.0393

MarkerSeek score 67.20

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 78
variable_sites: 14
pi: 0.0393
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGAGTTGTTCGATTTTTCCCA	GTTTTATTCTTCGTCGCCGT	38.1	45.0	56.0	56.9	169-182 bp	1.0	58.6
rps19-rpl2_p2	TGAGTTGTTCGATTTTTCCCA	AGTTTTATTCTTCGTCGCCGT	38.1	42.9	56.0	58.3	170-183 bp	1.0	57.2
rps19-rpl2_p3	TGAGTTGTTCGATTTTTCCCAA	GTTTTATTCTTCGTCGCCGT	36.4	45.0	56.7	56.9	169-182 bp	1.0	56.5
rps19-rpl2_p4	TTGAGTTGTTCGATTTTTCCCA	GTTTTATTCTTCGTCGCCGT	36.4	45.0	56.7	56.9	170-183 bp	1.0	56.4
rps19-rpl2_p5	TGAGTTGTTCGATTTTTCCCA	AGTTTTATTCTTCGTCGCCG	38.1	45.0	56.0	56.6	170-183 bp	1.0	56.2