Hotspot

rpl22-rps19

LSC · 86836-87161 · 326 bp

Back to Ottelia

Pi 0.0252

MarkerSeek score 62.90

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 326
variable_sites: 33
pi: 0.0252
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TTTCTTCATAGGAACGCCCG	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	1009-1068 bp	1.0	89.0
rpl22-rps19_p2	TTTCTTCATAGGAACGCCCG	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1010-1069 bp	1.0	89.0
rpl22-rps19_p3	ACCTTTTCACAATGGGACCC	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	1168-1227 bp	1.0	88.8
rpl22-rps19_p4	ACCTTTTCACAATGGGACCC	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1169-1228 bp	1.0	88.8
rpl22-rps19_p5	TTCGATTCCTCGTGTTCGAC	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	1607-1666 bp	1.0	87.9