Hotspot

petA-psbJ

LSC · 65835-66788 · 954 bp

Back to Ottelia

Pi 0.0201

MarkerSeek score 62.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 954
variable_sites: 77
pi: 0.0201
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCGAATTAGCTCCTCCCGAT	AAAGCAAGGTAAATGGCCGA	50.0	45.0	58.0	58.1	1688-1715 bp	1.0	87.9
petA-psbJ_p2	TCGAATTAGCTCCTCCCGAT	TGGACTAGCTGTACCTACCG	50.0	55.0	58.0	58.0	2018-2045 bp	1.0	87.0
petA-psbJ_p3	TCGAATTAGCTCCTCCCGAT	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	2017-2044 bp	1.0	87.0
petA-psbJ_p4	TCGAATTAGCTCCTCCCGAT	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	2249-2276 bp	1.0	86.3
petA-psbJ_p5	TCGAATTAGCTCCTCCCGAT	CGGGTTGGTTATTCGTCAGT	50.0	50.0	58.0	57.9	2236-2263 bp	1.0	86.1