Hotspot

ycf3-trnS-GGA

LSC · 46242-46888 · 647 bp

Back to Ottelia

Pi 0.0148

MarkerSeek score 64.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 647
variable_sites: 43
pi: 0.0148
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	CTACACCTTCAACCACTCGG	50.0	55.0	57.1	57.9	420-799 bp	1.0	81.0
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	CTACACCTTCAACCACTCGG	50.0	55.0	57.1	57.9	419-798 bp	1.0	81.0
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	457-836 bp	1.0	80.7
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	456-835 bp	1.0	80.7
ycf3-trnS-GGA_p5	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	478-857 bp	1.0	80.1