Hotspot

trnS-GCU-trnG-UCC

LSC · 8592-9542 · 951 bp

Back to Ottelia

Pi 0.0231
MarkerSeek score 78.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
951
variable_sites
108
pi
0.0231
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAACAAAACTGAAAGACCCCT 50.0 40.9 58.0 58.0 1050-1098 bp 0.894737 71.4
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAACAAAACTGAAAGACCCCT 50.0 40.9 58.0 58.0 1049-1097 bp 0.894737 71.4
trnS-GCU-trnG-UCC_p3 GATCAGAGGGAACGGAGAGA TGAACAAAACTGAAAGACCCCT 55.0 40.9 57.9 58.0 1115-1162 bp 0.842105 67.9
trnS-GCU-trnG-UCC_p4 ATCAGAGGGAACGGAGAGAG TGAACAAAACTGAAAGACCCCT 55.0 40.9 57.9 58.0 1114-1161 bp 0.842105 67.9
trnS-GCU-trnG-UCC_p5 CTCAAGCGATCAGAGGGAAC TGAACAAAACTGAAAGACCCCT 55.0 40.9 58.1 58.0 1122-1169 bp 0.842105 67.8