Hotspot

trnfM-CAU

LSC · 38237-38293 · 57 bp

Back to Otatea

Pi 0.0175
MarkerSeek score 62.60
Primer available yes
Label trnfM-CAU

Feature Metadata

label_name
trnfM-CAU
feature_type
tRNA
region
LSC
length_bp
57
variable_sites
1
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU_p1 AGAAGGAATATGGAGCGGGT CCCCGCTCTTTCGTTTAAGA 50.0 50.0 58.2 57.9 103 bp 1.0 78.5
trnfM-CAU_p2 AGAAGGAATATGGAGCGGGT TCCCCGCTCTTTCGTTTAAG 50.0 50.0 58.2 57.9 104 bp 1.0 78.5
trnfM-CAU_p3 AGAAGGAATATGGAGCGGGT GTTCCCCGCTCTTTCGTTTA 50.0 50.0 58.2 58.2 106 bp 1.0 77.6
trnfM-CAU_p4 AGAAGGAATATGGAGCGGGT TATGGTAGAGGGGCGTATCC 50.0 55.0 58.2 57.8 145 bp 1.0 77.2
trnfM-CAU_p5 AGGAATATGGAGCGGGTAGT CCCCGCTCTTTCGTTTAAGA 50.0 50.0 57.6 57.9 100 bp 1.0 76.9