Hotspot

trnT-GGU

LSC · 15976-16042 · 67 bp

Back to Otatea

Pi 0.0299
MarkerSeek score 69.70
Primer available yes
Label trnT-GGU

Feature Metadata

label_name
trnT-GGU
feature_type
tRNA
region
LSC
length_bp
67
variable_sites
2
pi
0.0299
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU_p1 AGCCCTTTTAACTCAGTGGT ACCAAAAATGCATGCATCCC 45.0 45.0 56.7 57.6 247 bp 1.0 66.6
trnT-GGU_p2 AGGCATAAGTCATCGGTTCA ACCAAAAATGCATGCATCCC 45.0 45.0 56.6 57.6 210 bp 1.0 66.5
trnT-GGU_p3 AGCCCTTTTAACTCAGTGGT GCATCCCGCACTAAGTCATA 45.0 50.0 56.7 57.5 234 bp 1.0 65.7
trnT-GGU_p4 TGGTAAGGCATAAGTCATCGG ACCAAAAATGCATGCATCCC 47.6 45.0 57.5 57.6 215 bp 1.0 65.7
trnT-GGU_p5 GGTAAGGCATAAGTCATCGGT ACCAAAAATGCATGCATCCC 47.6 45.0 57.5 57.6 214 bp 1.0 65.7