Hotspot

rps16

LSC · 4519-5645 · 1127 bp

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Pi 0
MarkerSeek score 60.70
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1127
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACAGCGAGGGATACTAGTGA TCCCTATACCACTTTGCAATGA 50.0 40.9 57.3 57.1 1201-1224 bp 1.0 52.8
rps16_p2 AGACAGCGAGGGATACTAGT TCCCTATACCACTTTGCAATGA 50.0 40.9 57.0 57.1 1203-1226 bp 1.0 50.4
rps16_p3 ACAGCGAGGGATACTAGTGA TCAATTCCCTATACCACTTTGCA 50.0 39.1 57.3 57.8 1206-1229 bp 1.0 50.2
rps16_p4 ACAGCGAGGGATACTAGTGA TCCCTATACCACTTTGCAATGAA 50.0 39.1 57.3 57.8 1201-1224 bp 1.0 50.2
rps16_p5 ACAGCGAGGGATACTAGTGA TTCCCTATACCACTTTGCAATGA 50.0 39.1 57.3 57.8 1202-1225 bp 1.0 50.2