Hotspot

ndhH

IRb · 102946-103167 · 222 bp

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Pi 0.018
MarkerSeek score 48.50
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
IRb
length_bp
222
variable_sites
4
pi
0.018
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 ATCCCGTGAAATTCTCGAGC GCCCCTTATTCCCCTTCCTA 50.0 55.0 58.1 58.2 1002-1060 bp 1.0 78.6
ndhH_p2 ATCCCGTGAAATTCTCGAGC TAGGGGGAGGACGAATATCG 50.0 55.0 58.1 57.8 1115-1173 bp 1.0 78.3
ndhH_p3 TGCATTTTGATGAGCCTCGT GCCCCTTATTCCCCTTCCTA 45.0 55.0 58.2 58.2 348-406 bp 1.0 78.3
ndhH_p4 TGCATTTTGATGAGCCTCGT TAGGGGGAGGACGAATATCG 45.0 55.0 58.2 57.8 461-519 bp 1.0 77.7
ndhH_p5 ATTCTCGAGCCGAAAGATGG GCCCCTTATTCCCCTTCCTA 50.0 55.0 57.8 58.2 992-1050 bp 1.0 77.2