Hotspot

psbE-petL

LSC · 64469-65710 · 1242 bp

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Pi 0
MarkerSeek score 60.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1242
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AAGCAGCCAGTAGAAAACCG 45.0 50.0 58.0 58.5 1377-1400 bp 1.0 77.4
psbE-petL_p2 TGACAAATAACCAACCCGCA GCAGCCAGTAGAAAACCGAA 45.0 50.0 58.0 58.5 1375-1398 bp 1.0 77.3
psbE-petL_p3 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 50.0 50.0 57.9 58.5 1366-1389 bp 1.0 77.1
psbE-petL_p4 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 50.0 50.0 57.9 58.5 1364-1387 bp 1.0 77.1
psbE-petL_p5 ACCAACCCGCAATGAATAGG AAGCAGCCAGTAGAAAACCG 50.0 50.0 58.2 58.5 1368-1391 bp 1.0 75.6