Hotspot

psaA-ycf3

LSC · 43413-44026 · 614 bp

Back to Otatea

Pi 0.0147
MarkerSeek score 67.20
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
614
variable_sites
9
pi
0.0147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TGTTCTTGAGAAATGGCCGG TATAGCGCTTACTCCGGGAA 50.0 50.0 58.5 58.0 795 bp 1.0 77.1
psaA-ycf3_p2 TTTTACTTCTGGTTCCGGCG TATAGCGCTTACTCCGGGAA 50.0 50.0 58.5 58.0 723 bp 1.0 77.0
psaA-ycf3_p3 TGTTCTTGAGAAATGGCCGG GCGCTTACTCCGGGAAATTA 50.0 50.0 58.5 58.1 791 bp 1.0 76.7
psaA-ycf3_p4 TTTTACTTCTGGTTCCGGCG GCGCTTACTCCGGGAAATTA 50.0 50.0 58.5 58.1 719 bp 1.0 76.7
psaA-ycf3_p5 GGTTTGGCCCATTCCTCAAA TATAGCGCTTACTCCGGGAA 50.0 50.0 58.7 58.0 776 bp 1.0 75.6