Hotspot

psbZ-trnfM-CAU

LSC · 12216-13001 · 786 bp

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Pi 0.0179
MarkerSeek score 69.40
Primer available yes
Label psbZ-trnfM-CAU

Feature Metadata

label_name
psbZ-trnfM-CAU
feature_type
igs
region
LSC
length_bp
786
variable_sites
14
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnfM-CAU_p1 TTGCTTCTCCTGATGGTTGG CGAGGCTCATAACCTTGAGG 50.0 55.0 57.8 57.8 943-948 bp 1.0 77.9
psbZ-trnfM-CAU_p2 GCTTCTCCTGATGGTTGGTC CGAGGCTCATAACCTTGAGG 55.0 55.0 58.3 57.8 941-946 bp 1.0 77.5
psbZ-trnfM-CAU_p3 TTGCTTCTCCTGATGGTTGG CAGTTTGGTAGCTCACGAGG 50.0 55.0 57.8 58.3 958-963 bp 1.0 77.5
psbZ-trnfM-CAU_p4 GCTTCTCCTGATGGTTGGTC CAGTTTGGTAGCTCACGAGG 55.0 55.0 58.3 58.3 956-961 bp 1.0 77.1
psbZ-trnfM-CAU_p5 TTGCTTCTCCTGATGGTTGG GAGCGGAGTAGAGCAGTTTG 50.0 55.0 57.8 58.4 971-976 bp 1.0 76.8