Hotspot

psbK-psbI

LSC · 7316-7714 · 399 bp

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Pi 0.0175
MarkerSeek score 57.00
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
399
variable_sites
7
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 CGTGGATGTTATGCCTGTCA GGATTACGTCCTGGGTCATT 50.0 50.0 58.0 57.0 573-579 bp 1.0 72.8
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG GGATTACGTCCTGGGTCATT 50.0 50.0 57.9 57.0 524-530 bp 1.0 72.6
psbK-psbI_p3 TGGCAAGCTGCTGTAAGTTT GGATTACGTCCTGGGTCATT 45.0 50.0 58.3 57.0 521-527 bp 1.0 70.6
psbK-psbI_p4 TTGGCAAGCTGCTGTAAGTT GGATTACGTCCTGGGTCATT 45.0 50.0 58.3 57.0 522-528 bp 1.0 70.6
psbK-psbI_p5 TTTGGCAAGCTGCTGTAAGT GGATTACGTCCTGGGTCATT 45.0 50.0 58.3 57.0 523-529 bp 1.0 70.6