Hotspot

APL01_gp013-APL01_gp012

Genome · 93516-93595 · 80 bp

Back to Osyris

Pi 0.0132
MarkerSeek score 64.80
Primer available yes
Label APL01_gp013-APL01_gp012

Feature Metadata

label_name
APL01_gp013-APL01_gp012
feature_type
igs
region
Genome
length_bp
80
variable_sites
1
pi
0.0132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gp013-APL01_gp012_p1 ATGGCTTTTCCCGGATGAAA AACAGGTGTTTCCACGACTC 45.0 50.0 57.8 58.1 145-150 bp 1.0 81.0
APL01_gp013-APL01_gp012_p2 TGGCTTTTCCCGGATGAAAT AACAGGTGTTTCCACGACTC 45.0 50.0 57.8 58.1 144-149 bp 1.0 81.0
APL01_gp013-APL01_gp012_p3 AAGATGGCTTTTCCCGGATG AACAGGTGTTTCCACGACTC 50.0 50.0 58.2 58.1 148-153 bp 1.0 80.9
APL01_gp013-APL01_gp012_p4 GATGGCTTTTCCCGGATGAA AACAGGTGTTTCCACGACTC 50.0 50.0 58.2 58.1 146-151 bp 1.0 80.9
APL01_gp013-APL01_gp012_p5 GTTCCTATCTAACGGAGCGC AACAGGTGTTTCCACGACTC 55.0 50.0 58.2 58.1 202-207 bp 1.0 80.1