Hotspot

APL01_gp020-APL01_gp019

Genome · 81255-81375 · 121 bp

Back to Osyris

Pi 0.0496
MarkerSeek score 70.60
Primer available yes
Label APL01_gp020-APL01_gp019

Feature Metadata

label_name
APL01_gp020-APL01_gp019
feature_type
igs
region
Genome
length_bp
121
variable_sites
6
pi
0.0496
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gp020-APL01_gp019_p1 CCGCTATTGTCCGCTACATT AATAGCGGCGTCAAAAATGC 50.0 45.0 58.1 58.1 217-224 bp 1.0 80.4
APL01_gp020-APL01_gp019_p2 TCCGCTACATTCAAATGGGT AATAGCGGCGTCAAAAATGC 45.0 45.0 57.2 58.1 208-215 bp 1.0 75.1
APL01_gp020-APL01_gp019_p3 CCGCTATTGTCCGCTACATT CATTTCAATAGCGGCGTCAA 50.0 45.0 58.1 57.2 223-230 bp 1.0 74.5
APL01_gp020-APL01_gp019_p4 TACACATCAACTCTCGAGCC AATAGCGGCGTCAAAAATGC 50.0 45.0 57.1 58.1 237-244 bp 1.0 73.7
APL01_gp020-APL01_gp019_p5 CCGCTATTGTCCGCTACATT CCATTTCAATAGCGGCGTCA 50.0 50.0 58.1 59.0 224-231 bp 1.0 73.1