Hotspot

APL01_gp042-APL01_gp041

Genome · 64496-64517 · 22 bp

Back to Osyris

Pi 0.0455
MarkerSeek score 78.60
Primer available yes
Label APL01_gp042-APL01_gp041

Feature Metadata

label_name
APL01_gp042-APL01_gp041
feature_type
igs
region
Genome
length_bp
22
variable_sites
1
pi
0.0455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gp042-APL01_gp041_p1 TTCGGATTTGATTGTGTCGT ACGGACTAGCTGTACCTACC 40.0 55.0 55.7 58.0 110 bp 1.0 61.9
APL01_gp042-APL01_gp041_p2 TTCGGATTTGATTGTGTCGT GGACTAGCTGTACCTACCGT 40.0 55.0 55.7 58.0 108 bp 1.0 61.9
APL01_gp042-APL01_gp041_p3 CATTCGGATTTGATTGTGTCGT ACGGACTAGCTGTACCTACC 40.9 55.0 57.7 58.0 112 bp 1.0 61.6
APL01_gp042-APL01_gp041_p4 CATTCGGATTTGATTGTGTCGT GGACTAGCTGTACCTACCGT 40.9 55.0 57.7 58.0 110 bp 1.0 61.6
APL01_gp042-APL01_gp041_p5 TTCGGATTTGATTGTGTCGT ATGGTTAGCTGTTCACGGAC 40.0 50.0 55.7 57.9 124 bp 1.0 61.5