Hotspot

APL01_gp067-APL01_gp066

Genome · 14212-15286 · 1075 bp

Back to Osyris

Pi 0.00938
MarkerSeek score 64.90
Primer available yes
Label APL01_gp067-APL01_gp066

Feature Metadata

label_name
APL01_gp067-APL01_gp066
feature_type
igs
region
Genome
length_bp
1075
variable_sites
10
pi
0.00938
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gp067-APL01_gp066_p1 TACCTTCTACAGCTTGGCCT GCGAATCTTTGGAGGGTCAT 50.0 50.0 58.0 58.0 1216-1242 bp 1.0 80.5
APL01_gp067-APL01_gp066_p2 CAGTACCTTGACCAACTCCG GCGAATCTTTGGAGGGTCAT 55.0 50.0 57.9 58.0 1192-1218 bp 1.0 80.3
APL01_gp067-APL01_gp066_p3 AACGGAAGCAGCAGAAATCA GCGAATCTTTGGAGGGTCAT 45.0 50.0 58.1 58.0 1131-1157 bp 1.0 80.1
APL01_gp067-APL01_gp066_p4 TACCTTCTACAGCTTGGCCT TTTTGCAACTTTAGCTGCGG 50.0 45.0 58.0 57.9 1245-1271 bp 1.0 79.8
APL01_gp067-APL01_gp066_p5 AGCCAATCCAGCAGCAATAA GCGAATCTTTGGAGGGTCAT 45.0 50.0 57.9 58.0 1149-1175 bp 1.0 79.8