Hotspot

APL01_gt004-APL01_gt005

Genome · 9115-9674 · 560 bp

Back to Osyris

Pi 0.00893
MarkerSeek score 63.10
Primer available yes
Label APL01_gt004-APL01_gt005

Feature Metadata

label_name
APL01_gt004-APL01_gt005
feature_type
igs
region
Genome
length_bp
560
variable_sites
5
pi
0.00893
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gt004-APL01_gt005_p1 CAATCCACCGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 57.9 58.2 629-630 bp 1.0 69.9
APL01_gt004-APL01_gt005_p2 AATCCACCGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 57.9 58.2 628-629 bp 1.0 69.9
APL01_gt004-APL01_gt005_p3 CAATCCACCGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 57.9 56.8 629-630 bp 1.0 69.8
APL01_gt004-APL01_gt005_p4 AATCCACCGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 57.9 56.8 628-629 bp 1.0 69.8
APL01_gt004-APL01_gt005_p5 CTCGTACAGCGGATTAGCAA ACGAATCGCACTTTTACCACT 50.0 42.9 57.8 58.2 646-647 bp 1.0 69.1