Hotspot

APL01_gp033

Genome · 69976-133316 · 63341 bp

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Pi 0.00547
MarkerSeek score 47.80
Primer available yes
Label APL01_gp033

Feature Metadata

label_name
APL01_gp033
feature_type
gene
region
Genome
length_bp
63341
variable_sites
346
pi
0.00547
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gp033_p1 CATGGGTCGAGAACGGAAAT CCTTGTTCCTACATGACCCG 50.0 55.0 58.0 58.0 1453-1455 bp 1.0 81.8
APL01_gp033_p2 TCCACAGTCCTGAGGCTAAA CCTTGTTCCTACATGACCCG 50.0 55.0 58.0 58.0 1144-1151 bp 1.0 81.8
APL01_gp033_p3 TTCCACAGTCCTGAGGCTAA CCTTGTTCCTACATGACCCG 50.0 55.0 58.0 58.0 1145-1152 bp 1.0 81.8
APL01_gp033_p4 TGAGATCAAATCCACCGCTG CCTTGTTCCTACATGACCCG 50.0 55.0 58.0 58.0 1423-1425 bp 1.0 81.8
APL01_gp033_p5 GAGATCAAATCCACCGCTGT CCTTGTTCCTACATGACCCG 50.0 55.0 58.0 58.0 1422-1424 bp 1.0 81.8