Hotspot

APL01_gp032

Genome · 1-70089 · 70089 bp

Back to Osyris

Pi 0.00567
MarkerSeek score 55.90
Primer available yes
Label APL01_gp032

Feature Metadata

label_name
APL01_gp032
feature_type
gene
region
Genome
length_bp
70089
variable_sites
397
pi
0.00567
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APL01_gp032_p1 CAATCCACTGCCTTGATCCA GAGGTATTGAAGGTAAAGGAGCA 50.0 43.5 57.9 57.9 331-338 bp 1.0 58.2
APL01_gp032_p2 AATCCACTGCCTTGATCCAC GAGGTATTGAAGGTAAAGGAGCA 50.0 43.5 57.9 57.9 330-337 bp 1.0 58.2
APL01_gp032_p3 ACAATCCACTGCCTTGATCC GAGGTATTGAAGGTAAAGGAGCA 50.0 43.5 57.9 57.9 332-339 bp 1.0 58.2
APL01_gp032_p4 CTTGATCCACTTGGCCACAT GAGGTATTGAAGGTAAAGGAGCA 50.0 43.5 58.2 57.9 320-327 bp 1.0 58.2
APL01_gp032_p5 TTGATCCACTTGGCCACATC GAGGTATTGAAGGTAAAGGAGCA 50.0 43.5 58.2 57.9 319-326 bp 1.0 58.1