Hotspot

ycf1

SSC · 128663-134401 · 5739 bp

Back to Ostryopsis

Pi 0.00436
MarkerSeek score 50.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5739
variable_sites
25
pi
0.00436
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCCAATGATTATGCAGTTCA ATGGAATCGCCCATTTCGAT 35.0 45.0 52.5 57.7 221 bp 1.0 41.8
ycf1_p2 TTCCAATGATTATGCAGTTCA ATGGAATCGCCCATTTCGAT 33.3 45.0 53.5 57.7 222 bp 1.0 41.8
ycf1_p3 TCCAATGATTATGCAGTTCA GATGGAATCGCCCATTTCGA 35.0 50.0 52.5 58.4 222 bp 1.0 41.8
ycf1_p4 TTCCAATGATTATGCAGTTCA GATGGAATCGCCCATTTCGA 33.3 50.0 53.5 58.4 223 bp 1.0 41.8
ycf1_p5 TCCAATGATTATGCAGTTCAT ATGGAATCGCCCATTTCGAT 33.3 45.0 53.2 57.7 221 bp 1.0 41.8