Pi
0.059
MarkerSeek score
82.40
Primer available
yes
Label
rrn4.5
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rrn4.5_p1 | GGATGGAGCGACAGAAGTTT | TGTGAGGCCCAATTCTTGAC | 50.0 | 50.0 | 57.9 | 58.1 | 350 bp | 1.0 | 82.1 |
| rrn4.5_p2 | GGATGGAGCGACAGAAGTTT | TTGGCTAGTGATTGTGAGGC | 50.0 | 50.0 | 57.9 | 57.9 | 362 bp | 1.0 | 81.8 |
| rrn4.5_p3 | TCTCCTATTCCGACTTCCCC | TGTGAGGCCCAATTCTTGAC | 55.0 | 50.0 | 57.9 | 58.1 | 421 bp | 1.0 | 81.6 |
| rrn4.5_p4 | TCTCCTATTCCGACTTCCCC | TTGGCTAGTGATTGTGAGGC | 55.0 | 50.0 | 57.9 | 57.9 | 433 bp | 1.0 | 81.4 |
| rrn4.5_p5 | TCTCCTATTCCGACTTCCCC | CCAATTCTTGACTGGAGGGG | 55.0 | 55.0 | 57.9 | 57.9 | 413 bp | 1.0 | 81.4 |
| rrn4.5_p1 | TGTGAGGCCCAATTCTTGAC | GGATGGAGCGACAGAAGTTT | 50.0 | 50.0 | 58.1 | 57.9 | 350 bp | 1.0 | 82.1 |
| rrn4.5_p2 | TTGGCTAGTGATTGTGAGGC | GGATGGAGCGACAGAAGTTT | 50.0 | 50.0 | 57.9 | 57.9 | 362 bp | 1.0 | 81.8 |
| rrn4.5_p3 | TGTGAGGCCCAATTCTTGAC | TCTCCTATTCCGACTTCCCC | 50.0 | 55.0 | 58.1 | 57.9 | 421 bp | 1.0 | 81.6 |
| rrn4.5_p4 | TTGGCTAGTGATTGTGAGGC | TCTCCTATTCCGACTTCCCC | 50.0 | 55.0 | 57.9 | 57.9 | 433 bp | 1.0 | 81.4 |
| rrn4.5_p5 | CCAATTCTTGACTGGAGGGG | TCTCCTATTCCGACTTCCCC | 55.0 | 55.0 | 57.9 | 57.9 | 413 bp | 1.0 | 81.4 |