Hotspot

petL-petG

LSC · 70189-70368 · 180 bp

Back to Ostryopsis

Pi 0.0115

MarkerSeek score 62.30

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 2
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TCGGTTTTCTACTAGCGGTT	GATCGCCACGTCTGTATTGT	45.0	50.0	56.6	58.1	348-354 bp	1.0	70.0
petL-petG_p2	TTCGGTTTTCTACTAGCGGT	GATCGCCACGTCTGTATTGT	45.0	50.0	56.6	58.1	349-355 bp	1.0	70.0
petL-petG_p3	TCGGTTTTCTACTAGCGGTT	TGATCGCCACGTCTGTATTG	45.0	50.0	56.6	58.1	349-355 bp	1.0	70.0
petL-petG_p4	TTCGGTTTTCTACTAGCGGT	TGATCGCCACGTCTGTATTG	45.0	50.0	56.6	58.1	350-356 bp	1.0	70.0
petL-petG_p5	TCGGTTTTCTACTAGCGGTT	TCAAAGGTCCAACTGATCGC	45.0	50.0	56.6	58.2	362-368 bp	1.0	69.0