Hotspot

accD-psaI

LSC · 62832-63738 · 907 bp

Back to Ostryopsis

Pi 0.00225

MarkerSeek score 62.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 2
pi: 0.00225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GATCCAATCGTACCGCGTAA	AAGAAACCATCGCAATTGCC	50.0	45.0	57.9	57.6	1064-1082 bp	1.0	77.6
accD-psaI_p2	TCCAATCGTACCGCGTAATC	AAGAAACCATCGCAATTGCC	50.0	45.0	57.9	57.6	1062-1080 bp	1.0	77.6
accD-psaI_p3	CCAATCGTACCGCGTAATCT	AAGAAACCATCGCAATTGCC	50.0	45.0	57.9	57.6	1061-1079 bp	1.0	77.6
accD-psaI_p4	TCCAATCGTACCGCGTAATC	ATCGCAATTGCCGGAAAGAA	50.0	45.0	57.9	58.8	1054-1072 bp	1.0	74.0
accD-psaI_p5	TCCAATCGTACCGCGTAATC	TCGCAATTGCCGGAAAGAAT	50.0	45.0	57.9	58.8	1053-1071 bp	1.0	74.0