Hotspot

trnF-GAA-ndhJ

LSC · 52271-53090 · 820 bp

Back to Ostryopsis

Pi 0.00732

MarkerSeek score 63.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 6
pi: 0.00732
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	971-988 bp	1.0	77.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	935-952 bp	1.0	77.3
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	959-976 bp	1.0	76.6
trnF-GAA-ndhJ_p4	AAGATAAGGATGATGCGCCG	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.2	57.7	1000-1017 bp	1.0	76.1
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GCCGGAAAGTTGGATAGGAT	55.0	50.0	58.0	57.4	969-986 bp	1.0	75.3