Hotspot

atpA-atpF

LSC · 13363-13425 · 63 bp

Back to Ostryopsis

Pi 0.0635

MarkerSeek score 82.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 4
pi: 0.0635
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	TGTTTGGGACGCTGAAAGAA	55.0	45.0	58.1	58.0	208 bp	1.0	80.4
atpA-atpF_p2	GTCGCCTACTTGAAGTACGG	TGTTTGGGACGCTGAAAGAA	55.0	45.0	58.1	58.0	206 bp	1.0	80.4
atpA-atpF_p3	CCGTCGCCTACTTGAAGTAC	ATGTTTGGGACGCTGAAAGA	55.0	45.0	58.1	57.7	209 bp	1.0	78.6
atpA-atpF_p4	GTCGCCTACTTGAAGTACGG	ATGTTTGGGACGCTGAAAGA	55.0	45.0	58.1	57.7	207 bp	1.0	78.6
atpA-atpF_p5	ACTTGAAGTACGGTACCGGT	TGTTTGGGACGCTGAAAGAA	50.0	45.0	58.4	58.0	199 bp	1.0	78.2