Hotspot

trnS-GCT-trnG-UCC

LSC · 9523-10396 · 874 bp

Back to Ostryopsis

Pi 0.00594
MarkerSeek score 61.90
Primer available yes
Label trnS-GCT-trnG-UCC

Feature Metadata

label_name
trnS-GCT-trnG-UCC
feature_type
igs
region
LSC
length_bp
874
variable_sites
5
pi
0.00594
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCT-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 931-943 bp 1.0 54.8
trnS-GCT-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 930-942 bp 1.0 54.8
trnS-GCT-trnG-UCC_p3 ACAAAAGCGGAAAGAGAGGG ACGAATCACACTTTTACCACT 50.0 38.1 58.1 55.5 991-1003 bp 1.0 53.9
trnS-GCT-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 934-946 bp 1.0 53.8
trnS-GCT-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 933-945 bp 1.0 53.8