Hotspot

trnQ-TTG-psbK

LSC · 8084-8577 · 494 bp

Back to Ostryopsis

Pi 0.00448

MarkerSeek score 62.60

Primer available yes

Label trnQ-TTG-psbK

Feature Metadata

label_name: trnQ-TTG-psbK
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 2
pi: 0.00448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-TTG-psbK_p1	ATAGGTATGCTCTGGGACGG	TAGGCTTCGGGTAATTTGGC	55.0	50.0	58.4	58.0	618-666 bp	1.0	80.9
trnQ-TTG-psbK_p2	GAATACCGGGACCAAAACCC	TAGGCTTCGGGTAATTTGGC	55.0	50.0	58.5	58.0	582-630 bp	1.0	79.6
trnQ-TTG-psbK_p3	ATAGGTATGCTCTGGGACGG	GCGTAGGCTTCGGGTAATTT	55.0	50.0	58.4	58.3	621-669 bp	1.0	78.6
trnQ-TTG-psbK_p4	GACCAAAACCCGTTGCCTTA	TAGGCTTCGGGTAATTTGGC	50.0	50.0	58.7	58.0	573-621 bp	1.0	78.5
trnQ-TTG-psbK_p5	ATAGGTATGCTCTGGGACGG	TCGGGTAATTTGGCGAAGAA	55.0	45.0	58.4	57.5	612-660 bp	1.0	77.5