Hotspot

ycf1

SSC · 128676-134426 · 5751 bp

Back to Ostrya

Pi 0.00174
MarkerSeek score 47.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5751
variable_sites
14
pi
0.00174
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGGGTCCGTTCAAGAAAGGA ACGTATCCACCGAGTTTATCA 50.0 42.9 58.6 56.6 280 bp 1.0 57.4
ycf1_p2 AGGGTCCGTTCAAGAAAGGA TCTTTTACGTATCCACCGAGT 50.0 42.9 58.6 56.5 286 bp 1.0 56.8
ycf1_p3 AGGGTCCGTTCAAGAAAGGA ACGTATCCACCGAGTTTATCAA 50.0 40.9 58.6 57.3 280 bp 1.0 55.1
ycf1_p4 AGGGTCCGTTCAAGAAAGGA TCTTTTACGTATCCACCGAGTT 50.0 40.9 58.6 57.2 286 bp 1.0 54.5
ycf1_p5 CGATAGGGTCCGTTCAAGAA ACGTATCCACCGAGTTTATCA 50.0 42.9 57.0 56.6 284 bp 1.0 54.3