Hotspot

psbC

LSC · 37162-38625 · 1464 bp

Back to Ostrya

Pi 0.0219
MarkerSeek score 45.90
Primer available yes
Label psbC

Feature Metadata

label_name
psbC
feature_type
gene
region
LSC
length_bp
1464
variable_sites
48
pi
0.0219
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC_p1 TCTTTGCGCCTAGTTTTGGT GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.0 58.1 2255 bp 1.0 82.0
psbC_p2 TGGTTCTTTGCGCCTAGTTT GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.0 58.1 2259 bp 1.0 82.0
psbC_p3 TTGGTTCTTTGCGCCTAGTT GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.0 58.1 2260 bp 1.0 82.0
psbC_p4 ACCGGTTTATGGATGAGTGC GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.0 58.1 1929 bp 1.0 82.0
psbC_p5 CTTTGCGCCTAGTTTTGGTG GGTTGATAGCTCCGGTCTTG 50.0 55.0 57.9 58.1 2254 bp 1.0 81.8