Hotspot

ndhA

SSC · 124497-126793 · 2297 bp

Pi 0.00145

MarkerSeek score 58.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2297
variable_sites: 5
pi: 0.00145
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCCCAGTTACCATAGGG	CCCGCCGGGTTTTATCAATT	55.0	50.0	58.1	58.9	2520-2536 bp	1.0	72.2
ndhA_p2	TGACCCCAGTTACCATAGGG	CCGCCGGGTTTTATCAATTT	55.0	45.0	58.1	57.0	2519-2535 bp	1.0	71.7
ndhA_p3	GACCCCAGTTACCATAGGGA	CCCGCCGGGTTTTATCAATT	55.0	50.0	57.8	58.9	2519-2535 bp	1.0	71.6
ndhA_p4	GACCCCAGTTACCATAGGGA	CCGCCGGGTTTTATCAATTT	55.0	45.0	57.8	57.0	2518-2534 bp	1.0	71.2
ndhA_p5	TGACCCCAGTTACCATAGGG	GCCGGGTTTTATCAATTTGCA	55.0	42.9	58.1	58.0	2517-2533 bp	1.0	71.1