Hotspot

trnN-GTT-ndhF

SSC · 112745-114417 · 1673 bp

Back to Ostrya

Pi 0.00279
MarkerSeek score 47.80
Primer available yes
Label trnN-GTT-ndhF

Feature Metadata

label_name
trnN-GTT-ndhF
feature_type
igs
region
SSC
length_bp
1673
variable_sites
7
pi
0.00279
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GTT-ndhF_p1 TGAACCTACGACCAGTCAGT ATATAGGGGGTGGCCGAATT 50.0 50.0 58.0 58.2 1828-1830 bp 1.0 78.4
trnN-GTT-ndhF_p2 ACGACCAGTCAGTTAACAGC ATATAGGGGGTGGCCGAATT 50.0 50.0 57.8 58.2 1821-1823 bp 1.0 77.2
trnN-GTT-ndhF_p3 TGAACCTACGACCAGTCAGT GGGTGGCCGAATTTCTTCTT 50.0 50.0 58.0 58.5 1821-1823 bp 1.0 76.7
trnN-GTT-ndhF_p4 TGAACCTACGACCAGTCAGT TATAGGGGGTGGCCGAATTT 50.0 50.0 58.0 58.5 1827-1829 bp 1.0 76.5
trnN-GTT-ndhF_p5 ACGACCAGTCAGTTAACAGC GGGTGGCCGAATTTCTTCTT 50.0 50.0 57.8 58.5 1814-1816 bp 1.0 75.6