Hotspot

psaJ-rpl33

LSC · 70955-71433 · 479 bp

Back to Ostrya

Pi 0.00278
MarkerSeek score 58.00
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
479
variable_sites
2
pi
0.00278
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TCCCGGATGCGTTGATATTC AACATCTTTACCCTTGGCCA 50.0 45.0 57.8 57.0 532 bp 1.0 71.1
psaJ-rpl33_p2 CCCGGATGCGTTGATATTCT AACATCTTTACCCTTGGCCA 50.0 45.0 57.8 57.0 531 bp 1.0 71.0
psaJ-rpl33_p3 TCCCGGATGCGTTGATATTC AGCACCATTTCGATTAGCACT 50.0 42.9 57.8 58.0 595 bp 1.0 70.9
psaJ-rpl33_p4 CCCGGATGCGTTGATATTCT AGCACCATTTCGATTAGCACT 50.0 42.9 57.8 58.0 594 bp 1.0 70.8
psaJ-rpl33_p5 TCCCGGATGCGTTGATATTC GAACATCTTTACCCTTGGCCA 50.0 47.6 57.8 58.2 533 bp 1.0 69.4