Hotspot

ndhC-trnM-CAT

LSC · 54336-55756 · 1421 bp

Back to Ostrya

Pi 0.00281
MarkerSeek score 60.60
Primer available yes
Label ndhC-trnM-CAT

Feature Metadata

label_name
ndhC-trnM-CAT
feature_type
igs
region
LSC
length_bp
1421
variable_sites
6
pi
0.00281
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAT_p1 GGGGCTAAAACTCCCGAAAT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.9 58.4 1562-1563 bp 1.0 76.0
ndhC-trnM-CAT_p2 CCTTTGCTAATCGGGGCTAA CCCGCCGTATGAAAGCAATA 50.0 50.0 57.7 58.4 1574-1575 bp 1.0 74.3
ndhC-trnM-CAT_p3 GGGGCTAAAACTCCCGAAAT CCGCCGTATGAAAGCAATAC 50.0 50.0 57.9 57.3 1561-1562 bp 1.0 73.9
ndhC-trnM-CAT_p4 GGGGCTAAAACTCCCGAAAT TTTGAACCAATGACTCCCGC 50.0 50.0 57.9 58.8 1577-1578 bp 1.0 73.2
ndhC-trnM-CAT_p5 CTTCTCTGGCCCTTTGCTAA CCCGCCGTATGAAAGCAATA 50.0 50.0 57.5 58.4 1584-1585 bp 1.0 73.1