Hotspot

trnT-TGT-trnF-GAA

LSC · 49984-51774 · 1791 bp

Back to Ostrya

Pi 0.00186
MarkerSeek score 59.70
Primer available yes
Label trnT-TGT-trnF-GAA

Feature Metadata

label_name
trnT-TGT-trnF-GAA
feature_type
igs
region
LSC
length_bp
1791
variable_sites
5
pi
0.00186
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-TGT-trnF-GAA_p1 AAAGCCTGCTATCGGAATCG TCTACCAGCTGAGCTATCCC 50.0 55.0 58.1 58.0 1889-1892 bp 1.0 79.4
trnT-TGT-trnF-GAA_p2 GCGATGCTCTAACCTCTGAG TCTACCAGCTGAGCTATCCC 55.0 55.0 57.9 58.0 1844-1847 bp 1.0 79.4
trnT-TGT-trnF-GAA_p3 AAAGCCTGCTATCGGAATCG TTTGAACTGGTGACACGAGG 50.0 50.0 58.1 58.1 1925-1928 bp 1.0 79.0
trnT-TGT-trnF-GAA_p4 GCGATGCTCTAACCTCTGAG TTTGAACTGGTGACACGAGG 55.0 50.0 57.9 58.1 1880-1883 bp 1.0 79.0
trnT-TGT-trnF-GAA_p5 AAAGCCTGCTATCGGAATCG GTGCCAGGAACCAGATTTGA 50.0 50.0 58.1 58.1 1940-1943 bp 1.0 78.7