Hotspot

trnE-TTC-trnT-GGT

LSC · 34156-34978 · 823 bp

Back to Ostrya

Pi 0.00972
MarkerSeek score 42.60
Primer available yes
Label trnE-TTC-trnT-GGT

Feature Metadata

label_name
trnE-TTC-trnT-GGT
feature_type
igs
region
LSC
length_bp
823
variable_sites
12
pi
0.00972
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-TTC-trnT-GGT_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 920-926 bp 1.0 79.4
trnE-TTC-trnT-GGT_p2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 899-905 bp 1.0 79.3
trnE-TTC-trnT-GGT_p3 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 899-905 bp 1.0 79.0
trnE-TTC-trnT-GGT_p4 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.7 58.1 878-884 bp 1.0 78.9
trnE-TTC-trnT-GGT_p5 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 896-902 bp 1.0 78.8