Hotspot

trnY-GTA-trnE-TTC

LSC · 34023-34082 · 60 bp

Back to Ostrya

Pi 0.0889
MarkerSeek score 63.00
Primer available yes
Label trnY-GTA-trnE-TTC

Feature Metadata

label_name
trnY-GTA-trnE-TTC
feature_type
igs
region
LSC
length_bp
60
variable_sites
8
pi
0.0889
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GTA-trnE-TTC_p1 TCTGGGGAACAACAAATGGG CATAGTATGATGGCGGTCGG 50.0 55.0 58.0 58.0 298 bp 0.666667 66.6
trnY-GTA-trnE-TTC_p2 GGATTTGGATCTGTCGGGAC CATAGTATGATGGCGGTCGG 55.0 55.0 58.0 58.0 820-821 bp 0.666667 66.6
trnY-GTA-trnE-TTC_p3 GCAAATGAACGGCGGTAAAA CATAGTATGATGGCGGTCGG 45.0 55.0 57.9 58.0 369 bp 0.666667 66.6
trnY-GTA-trnE-TTC_p4 TTCCGATCAGGCATTTCTGG CATAGTATGATGGCGGTCGG 50.0 55.0 58.0 58.0 313 bp 0.666667 66.3
trnY-GTA-trnE-TTC_p5 TCTGGGGAACAACAAATGGG ATACTTGCCCCCATCGTCTA 50.0 50.0 58.0 57.9 276 bp 0.666667 65.8