Hotspot

petN-psbM

LSC · 31183-32113 · 931 bp

Back to Ostrya

Pi 0.0043
MarkerSeek score 45.30
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
931
variable_sites
6
pi
0.0043
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1093 bp 1.0 68.0
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTC 50.0 45.0 58.2 56.1 1093 bp 1.0 63.8
petN-psbM_p3 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATTCT 50.0 42.9 57.1 57.6 1094 bp 1.0 62.0
petN-psbM_p4 CTCGCTTGGGCTGCTTTAAT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.9 57.6 1085 bp 1.0 62.0
petN-psbM_p5 ATGGGGTAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.0 57.6 1032 bp 1.0 61.6