Hotspot

atpH-atpI

LSC · 15401-16614 · 1214 bp

Pi 0.00247

MarkerSeek score 61.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1214
variable_sites: 4
pi: 0.00247
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1259-1301 bp	1.0	81.5
atpH-atpI_p2	GATCCCTTCTACAGCTTGGC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.0	57.9	1344-1386 bp	1.0	81.3
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1260-1302 bp	1.0	80.3
atpH-atpI_p4	CGATCCCTTCTACAGCTTGG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.8	57.9	1345-1387 bp	1.0	79.8
atpH-atpI_p5	ATAGAAGCAAGACCGACAGC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.7	57.9	1292-1334 bp	1.0	79.3