Hotspot

trnH-GTG-psbA

LSC · 84-492 · 409 bp

Back to Ostrya

Pi 0.00815
MarkerSeek score 60.00
Primer available yes
Label trnH-GTG-psbA

Feature Metadata

label_name
trnH-GTG-psbA
feature_type
igs
region
LSC
length_bp
409
variable_sites
5
pi
0.00815
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GTG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 525-531 bp 1.0 79.5
trnH-GTG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 539-545 bp 1.0 79.4
trnH-GTG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 538-544 bp 1.0 79.4
trnH-GTG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 540-546 bp 1.0 79.4
trnH-GTG-psbA_p5 TATTTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 58.1 58.0 586-592 bp 1.0 79.3