Hotspot

ycf1

IRb · 115095-117008 · 1914 bp

Back to Ostericum

Pi 0.119

MarkerSeek score 83.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1914
variable_sites: 455
pi: 0.119
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	TGTTCGTTGGATCCCTAGGA	55.0	50.0	58.0	57.8	2753-2864 bp	0.75	80.0
ycf1_p2	CCGGAACTTCTTCGTAGTGG	TGTTCGTTGGATCCCTAGGA	55.0	50.0	58.0	57.8	2752-2863 bp	0.75	80.0
ycf1_p3	CCCGGAACTTCTTCGTAGTG	GTGGGCGCATCTCTTCTTAT	55.0	50.0	58.0	57.8	2279-2390 bp	0.75	80.0
ycf1_p4	CCGGAACTTCTTCGTAGTGG	GTGGGCGCATCTCTTCTTAT	55.0	50.0	58.0	57.8	2278-2389 bp	0.75	80.0
ycf1_p5	ATCTCCCCAGGTAGGATTCG	TGTTCGTTGGATCCCTAGGA	55.0	50.0	58.0	57.8	2909-2996 bp	0.625	64.1