Hotspot

rpl32-trnL-UAG

SSC · 120453-121323 · 871 bp

Back to Ostericum

Pi 0.0129
MarkerSeek score 65.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
871
variable_sites
36
pi
0.0129
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGAAATCTCTTTCTACCGGA AGCGTGTCTACCGATTTCAC 47.6 50.0 56.8 58.0 963-1030 bp 1.0 68.1
rpl32-trnL-UAG_p2 GGGAAATCTCTTTCTACCGGA CAGCGTGTCTACCGATTTCA 47.6 50.0 56.8 58.0 964-1031 bp 1.0 68.1
rpl32-trnL-UAG_p3 AGGGAAATCTCTTTCTACCGG AGCGTGTCTACCGATTTCAC 47.6 50.0 56.8 58.0 964-1031 bp 1.0 68.0
rpl32-trnL-UAG_p4 AGGGAAATCTCTTTCTACCGG CAGCGTGTCTACCGATTTCA 47.6 50.0 56.8 58.0 965-1032 bp 1.0 68.0
rpl32-trnL-UAG_p5 AGCGTTGTCATTAGGGAAATCT AGCGTGTCTACCGATTTCAC 40.9 50.0 57.8 58.0 976-1043 bp 1.0 67.8