Hotspot

rpl22-rps19

LSC · 90352-90427 · 76 bp

Back to Ostericum

Pi 0.0685

MarkerSeek score 87.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 76
variable_sites: 13
pi: 0.0685
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GGACCGTCCACGAATTTGAT	AAATTTCCGAGGACACGCAA	50.0	45.0	58.3	58.4	217-257 bp	1.0	83.0
rpl22-rps19_p2	GGACCGTCCACGAATTTGAT	AATTTCCGAGGACACGCAAA	50.0	45.0	58.3	58.4	216-256 bp	1.0	83.0
rpl22-rps19_p3	GGACCGTCCACGAATTTGAT	ATTTCCGAGGACACGCAAAA	50.0	45.0	58.3	58.4	215-255 bp	1.0	83.0
rpl22-rps19_p4	GGACCGTCCACGAATTTGAT	TAAATTTCCGAGGACACGCA	50.0	45.0	58.3	57.5	218-258 bp	1.0	82.5
rpl22-rps19_p5	TAGGACCGTCCACGAATTTG	ATTTCCGAGGACACGCAAAA	50.0	45.0	57.6	58.4	217-257 bp	1.0	82.1