Hotspot

rbcL-accD

LSC · 63382-64008 · 627 bp

Back to Ostericum

Pi 0.0074

MarkerSeek score 63.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 627
variable_sites: 14
pi: 0.0074
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGGAGCCCTGAACTAGCT	AAACCGCCCGTTGATTTACT	50.0	45.0	58.4	58.1	781-796 bp	1.0	79.2
rbcL-accD_p2	AATGGAGCCCTGAACTAGCT	AACCGCCCGTTGATTTACTT	50.0	45.0	58.4	58.1	780-795 bp	1.0	79.2
rbcL-accD_p3	AAATGGAGCCCTGAACTAGC	AACCGCCCGTTGATTTACTT	50.0	45.0	57.6	58.1	781-796 bp	1.0	79.1
rbcL-accD_p4	AAATGGAGCCCTGAACTAGC	AAACCGCCCGTTGATTTACT	50.0	45.0	57.6	58.1	782-797 bp	1.0	79.0
rbcL-accD_p5	AATGGAGCCCTGAACTAGCT	TCAATAGGACCAAAACCGCC	50.0	50.0	58.4	58.2	793-808 bp	1.0	78.6