Hotspot

ndhC-trnV-UAC

LSC · 57098-58215 · 1118 bp

Back to Ostericum

Pi 0.0107

MarkerSeek score 60.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1118
variable_sites: 37
pi: 0.0107
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.9	58.1	929-1185 bp	1.0	46.6
ndhC-trnV-UAC_p2	ATCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	930-1186 bp	1.0	46.6
ndhC-trnV-UAC_p3	TCATATTCGTAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	929-1185 bp	1.0	46.6
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACA	AGTGCATGTTGGGTCTTTGA	31.8	45.0	53.9	57.6	993-1249 bp	1.0	46.5
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAGAAACA	GAGCCCAATGCACAAAAAGT	31.8	45.0	53.9	57.5	1010-1266 bp	1.0	46.4