Hotspot

psbZ-trnG-GCC

LSC · 42472-42767 · 296 bp

Back to Ostericum

Pi 0.00929

MarkerSeek score 62.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 296
variable_sites: 9
pi: 0.00929
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	449-456 bp	1.0	80.0
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	447-454 bp	1.0	79.6
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	TACCATTCGACCATATCCGC	50.0	50.0	57.8	57.3	422-429 bp	1.0	75.7
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	TACCATTCGACCATATCCGC	55.0	50.0	58.3	57.3	420-427 bp	1.0	75.3
psbZ-trnG-GCC_p5	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	443-450 bp	1.0	74.8