Hotspot

atpH-atpI

LSC · 13919-20475 · 6557 bp

Back to Ostericum

Pi 0.0381

MarkerSeek score 51.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 6557
variable_sites: 325
pi: 0.0381
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.18
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGGATCCAGTCAGCAATCC	CATCTGCCTGCTATCGATCC	55.0	55.0	58.0	58.0	1260-1297 bp	0.25	61.7
atpH-atpI_p2	GATCCAGTCAGCAATCCAGG	CATCTGCCTGCTATCGATCC	55.0	55.0	58.0	58.0	1257-1294 bp	0.25	61.7
atpH-atpI_p3	GGATCCAGTCAGCAATCCAG	CATCTGCCTGCTATCGATCC	55.0	55.0	58.0	58.0	1258-1295 bp	0.25	61.7
atpH-atpI_p4	CTTGTTTCAATCGGCTGCTG	CATCTGCCTGCTATCGATCC	50.0	55.0	58.0	58.0	803 bp	0.125	44.7
atpH-atpI_p5	TACCTAGCAAGCACAGCATG	CATCTGCCTGCTATCGATCC	50.0	55.0	58.0	58.0	763 bp	0.125	44.7