Hotspot

ycf2

IRb · 90256-97095 · 6840 bp

Back to Osteomeles

Pi 0.000146

MarkerSeek score 52.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6840
variable_sites: 1
pi: 0.000146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGTGGAAAAGGCTCGTATCA	CCCTTTCGTCACCATTTTGTG	45.0	47.6	56.9	58.0	197-203 bp	1.0	65.6
ycf2_p2	TGTGGAAAAGGCTCGTATCA	AGAAATGTCTGCGATCGAGT	45.0	45.0	56.9	57.1	280-286 bp	1.0	65.3
ycf2_p3	TGTGGAAAAGGCTCGTATCA	TCCCTTTCGTCACCATTTTGT	45.0	42.9	56.9	58.1	198-204 bp	1.0	65.2
ycf2_p4	TGTGGAAAAGGCTCGTATCAA	AGAAATGTCTGCGATCGAGT	42.9	45.0	57.6	57.1	280-286 bp	1.0	63.1
ycf2_p5	TGTGGAAAAGGCTCGTATCA	CAGAAATGTCTGCGATCGAG	45.0	50.0	56.9	56.7	281-287 bp	1.0	62.7