Hotspot

rpl16

LSC · 84922-86344 · 1423 bp

Back to Osteomeles

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1423
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCCAAAAACCCCTTTCGTT	TGGCAAACTCTTTCATTCTT	45.0	35.0	57.8	53.3	1562 bp	0.5	20.9
rpl16_p2	TCCCAAAAACCCCTTTCGTT	TGGCAAACTCTTTCATTCTTT	45.0	33.3	57.8	54.2	1562 bp	0.5	20.1
rpl16_p3	ATCCCAAAAACCCCTTTCGT	TGGCAAACTCTTTCATTCTT	45.0	35.0	57.6	53.3	1563 bp	0.5	20.0
rpl16_p4	TCCCAAAAACCCCTTTCGTT	ATGGCAAACTCTTTCATTCT	45.0	35.0	57.8	53.0	1563 bp	0.5	20.0
rpl16_p5	TCCCAAAAACCCCTTTCGTT	TGGCAAACTCTTTCATTCTTTT	45.0	31.8	57.8	55.0	1562 bp	0.5	20.0