Hotspot

psbA

LSC · 448-1509 · 1062 bp

Back to Osteomeles

Pi 0

MarkerSeek score 39.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGGAGCAATAACAAACCTCTTGA	AGAATTCGTGTGCTTGGGAG	39.1	50.0	58.0	58.2	1208 bp	1.0	54.4
psbA_p2	AGGAGCAATAACAAACCTCTTGA	AGAGAATTCGTGTGCTTGGG	39.1	50.0	58.0	58.2	1210 bp	1.0	54.4
psbA_p3	AGGAGCAATAACAAACCTCTTGA	GAGAATTCGTGTGCTTGGGA	39.1	50.0	58.0	58.2	1209 bp	1.0	54.4
psbA_p4	AGGAGCAATAACAAACCTCTTGA	AATTCGTGTGCTTGGGAGTC	39.1	50.0	58.0	58.5	1206 bp	1.0	52.1
psbA_p5	AGGAGCAATAACAAACCTCTTGA	GAATTCGTGTGCTTGGGAGT	39.1	50.0	58.0	58.5	1207 bp	1.0	52.1